7aad (Miltenyi Biotec)
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7aad
7aad, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/7-AAD+Staining+Solution/us12698315-681-21-22
Average 97 stars, based on 81 article reviews
7aad, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+solution/7-AAD+Staining+Solution/us12698315-681-21-22
Average 97 stars, based on 81 article reviews
7aad - by Bioz Stars,
2026-09
97/100 stars
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Flow Cytometry:Article Title: Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis. Article Snippet: .. Flow cytometry Surface marker staining of 5 105 freshly isolated monocytes or harvested hiPSC-derived monocyte-like cells was performed in 50 μl Article Title: A mechanical non-enzymatic method for isolation of mouse embryonic fibroblasts. Article Snippet: Mouse embryonic fibroblasts (MEFs) accessibility coupled with their simple generation make them as a typical embryonic cell model and feeder layer for in vitro expansion of pluripotent stem cells (PSCs).. In this study, a mechanical isolation technique was adopted to isolate MEFs and the efficiency of this technique was compared with enzymatic digestion method.. The suspended MEFs were prepared either by mechanical method or 0.25% trypsin enzymatic digestion. Marker:Article Title: Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis. Article Snippet: .. Flow cytometry Surface marker staining of 5 105 freshly isolated monocytes or harvested hiPSC-derived monocyte-like cells was performed in 50 μl Article Title: Focused ultrasound-induced blood-brain barrier opening promotes glioprotective phenotypes in ACSA-II+ murine astrocytes Article Snippet: .. First, the Staining:Article Title: Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis. Article Snippet: .. Flow cytometry Surface marker staining of 5 105 freshly isolated monocytes or harvested hiPSC-derived monocyte-like cells was performed in 50 μl Article Title: Focused ultrasound-induced blood-brain barrier opening promotes glioprotective phenotypes in ACSA-II+ murine astrocytes Article Snippet: .. First, the Article Title: Doxorubicin treatment modulates chemoresistance and affects the cell cycle in two canine mammary tumour cell lines Article Snippet: Aliquots of 1 × 10 6 cells were washed twice in 5 ml of PBS w/o Ca 2+ and Mg 2+ (Gibco-Life Technologies) and fixed overnight in 70% ice-cold ethanol (1 ml) added drop-by-drop with continuous vortexing. .. Then, the cells were washed with 10 ml of PBS and cellular pellet was incubated with 1 ml of Article Title: Comprehensive Evaluation of Biological Effects of Pentathiepins on Various Human Cancer Cell Lines and Insights into Their Mode of Action Article Snippet: .. The Article Title: Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116 Article Snippet: .. The suspension was stored at 4 °C for 24 h. After fixation, 1 mL of sample buffer was added while vortexing, followed by centrifugation at 17,000× g for 10 min. Article Title: Transcriptional CDK Inhibitors CYC065 and THZ1 Induce Apoptosis in Glioma Stem Cells Derived from Recurrent GBM Article Snippet: GBM cells were cultured as gliomaspheres and left untreated for basal expression analysis or treated with 150 μM TMZ (#T2577, Sigma-Aldrich, Arklow, Ireland), 3 μM CYC065 (#HY-101212, Medchemexpress, NJ, USA) or 100 nM THZ1 (#HY-80013, Medchemexpress, NJ, USA) for 120 h. At the end of treatment window, cells were harvested, dissociated using Accutase (Thermo Fisher Scientific, Waltham, MA, USA) and washed in FACS washing media (DPBS + 5% FCS, Gibco Life Technologies, Dún Laoghaire, Ireland). .. Cells were incubated for 15 min in FACS wash media on ice and stained with Article Title: Triple Selection Strategy for In Situ Labeling of Circulating Tumor Cells with High Purity and Viability toward Preclinical Personalized Drug Sensitivity Analysis. Article Snippet: DOI: 10.1002/adbi.202000013 clinical samples for analysis.. For example, identification and quantification of circulating tumor cells (CTCs)[1–3] or circulating tumor DNA (ctDNA)[4–6] from the blood samples have become complementary biomarkers to aid the selection of suitable treatment for patients.. [7] The enumeration analysis and molecular profiling of these extremely rare CTCs from patient blood samples (1–10 CTCs out of 109 blood cells mL−1) have been applied to the prostate, lung, breast, colon and other types of cancer. Article Title: A mechanical non-enzymatic method for isolation of mouse embryonic fibroblasts. Article Snippet: Mouse embryonic fibroblasts (MEFs) accessibility coupled with their simple generation make them as a typical embryonic cell model and feeder layer for in vitro expansion of pluripotent stem cells (PSCs).. In this study, a mechanical isolation technique was adopted to isolate MEFs and the efficiency of this technique was compared with enzymatic digestion method.. The suspended MEFs were prepared either by mechanical method or 0.25% trypsin enzymatic digestion. Isolation:Article Title: Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis. Article Snippet: .. Flow cytometry Surface marker staining of 5 105 freshly isolated monocytes or harvested hiPSC-derived monocyte-like cells was performed in 50 μl FACS:Article Title: Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis. Article Snippet: .. Flow cytometry Surface marker staining of 5 105 freshly isolated monocytes or harvested hiPSC-derived monocyte-like cells was performed in 50 μl Article Title: Transcriptional CDK Inhibitors CYC065 and THZ1 Induce Apoptosis in Glioma Stem Cells Derived from Recurrent GBM Article Snippet: GBM cells were cultured as gliomaspheres and left untreated for basal expression analysis or treated with 150 μM TMZ (#T2577, Sigma-Aldrich, Arklow, Ireland), 3 μM CYC065 (#HY-101212, Medchemexpress, NJ, USA) or 100 nM THZ1 (#HY-80013, Medchemexpress, NJ, USA) for 120 h. At the end of treatment window, cells were harvested, dissociated using Accutase (Thermo Fisher Scientific, Waltham, MA, USA) and washed in FACS washing media (DPBS + 5% FCS, Gibco Life Technologies, Dún Laoghaire, Ireland). .. Cells were incubated for 15 min in FACS wash media on ice and stained with Saline:Article Title: Efficient generation of osteoclasts from human induced pluripotent stem cells and functional investigations of lethal CLCN7-related osteopetrosis. Article Snippet: .. Flow cytometry Surface marker staining of 5 105 freshly isolated monocytes or harvested hiPSC-derived monocyte-like cells was performed in 50 μl Incubation:Article Title: Doxorubicin treatment modulates chemoresistance and affects the cell cycle in two canine mammary tumour cell lines Article Snippet: Aliquots of 1 × 10 6 cells were washed twice in 5 ml of PBS w/o Ca 2+ and Mg 2+ (Gibco-Life Technologies) and fixed overnight in 70% ice-cold ethanol (1 ml) added drop-by-drop with continuous vortexing. .. Then, the cells were washed with 10 ml of PBS and cellular pellet was incubated with 1 ml of Article Title: Comprehensive Evaluation of Biological Effects of Pentathiepins on Various Human Cancer Cell Lines and Insights into Their Mode of Action Article Snippet: .. The Article Title: Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116 Article Snippet: .. The suspension was stored at 4 °C for 24 h. After fixation, 1 mL of sample buffer was added while vortexing, followed by centrifugation at 17,000× g for 10 min. Article Title: Transcriptional CDK Inhibitors CYC065 and THZ1 Induce Apoptosis in Glioma Stem Cells Derived from Recurrent GBM Article Snippet: GBM cells were cultured as gliomaspheres and left untreated for basal expression analysis or treated with 150 μM TMZ (#T2577, Sigma-Aldrich, Arklow, Ireland), 3 μM CYC065 (#HY-101212, Medchemexpress, NJ, USA) or 100 nM THZ1 (#HY-80013, Medchemexpress, NJ, USA) for 120 h. At the end of treatment window, cells were harvested, dissociated using Accutase (Thermo Fisher Scientific, Waltham, MA, USA) and washed in FACS washing media (DPBS + 5% FCS, Gibco Life Technologies, Dún Laoghaire, Ireland). .. Cells were incubated for 15 min in FACS wash media on ice and stained with Article Title: Triple Selection Strategy for In Situ Labeling of Circulating Tumor Cells with High Purity and Viability toward Preclinical Personalized Drug Sensitivity Analysis. Article Snippet: DOI: 10.1002/adbi.202000013 clinical samples for analysis.. For example, identification and quantification of circulating tumor cells (CTCs)[1–3] or circulating tumor DNA (ctDNA)[4–6] from the blood samples have become complementary biomarkers to aid the selection of suitable treatment for patients.. [7] The enumeration analysis and molecular profiling of these extremely rare CTCs from patient blood samples (1–10 CTCs out of 109 blood cells mL−1) have been applied to the prostate, lung, breast, colon and other types of cancer. Article Title: A mechanical non-enzymatic method for isolation of mouse embryonic fibroblasts. Article Snippet: Mouse embryonic fibroblasts (MEFs) accessibility coupled with their simple generation make them as a typical embryonic cell model and feeder layer for in vitro expansion of pluripotent stem cells (PSCs).. In this study, a mechanical isolation technique was adopted to isolate MEFs and the efficiency of this technique was compared with enzymatic digestion method.. The suspended MEFs were prepared either by mechanical method or 0.25% trypsin enzymatic digestion. Suspension:Article Title: Comprehensive Evaluation of Biological Effects of Pentathiepins on Various Human Cancer Cell Lines and Insights into Their Mode of Action Article Snippet: .. The Article Title: Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116 Article Snippet: .. The suspension was stored at 4 °C for 24 h. After fixation, 1 mL of sample buffer was added while vortexing, followed by centrifugation at 17,000× g for 10 min. Centrifugation:Article Title: Berberine Inhibits Telomerase Activity and Induces Cell Cycle Arrest and Telomere Erosion in Colorectal Cancer Cell Line, HCT 116 Article Snippet: .. The suspension was stored at 4 °C for 24 h. After fixation, 1 mL of sample buffer was added while vortexing, followed by centrifugation at 17,000× g for 10 min. Control:Article Title: Transcriptional CDK Inhibitors CYC065 and THZ1 Induce Apoptosis in Glioma Stem Cells Derived from Recurrent GBM Article Snippet: GBM cells were cultured as gliomaspheres and left untreated for basal expression analysis or treated with 150 μM TMZ (#T2577, Sigma-Aldrich, Arklow, Ireland), 3 μM CYC065 (#HY-101212, Medchemexpress, NJ, USA) or 100 nM THZ1 (#HY-80013, Medchemexpress, NJ, USA) for 120 h. At the end of treatment window, cells were harvested, dissociated using Accutase (Thermo Fisher Scientific, Waltham, MA, USA) and washed in FACS washing media (DPBS + 5% FCS, Gibco Life Technologies, Dún Laoghaire, Ireland). .. Cells were incubated for 15 min in FACS wash media on ice and stained with Magnetic Cell Separation:Article Title: Transcriptional CDK Inhibitors CYC065 and THZ1 Induce Apoptosis in Glioma Stem Cells Derived from Recurrent GBM Article Snippet: GBM cells were cultured as gliomaspheres and left untreated for basal expression analysis or treated with 150 μM TMZ (#T2577, Sigma-Aldrich, Arklow, Ireland), 3 μM CYC065 (#HY-101212, Medchemexpress, NJ, USA) or 100 nM THZ1 (#HY-80013, Medchemexpress, NJ, USA) for 120 h. At the end of treatment window, cells were harvested, dissociated using Accutase (Thermo Fisher Scientific, Waltham, MA, USA) and washed in FACS washing media (DPBS + 5% FCS, Gibco Life Technologies, Dún Laoghaire, Ireland). .. Cells were incubated for 15 min in FACS wash media on ice and stained with |
